In Vitro Fertilization IVF

Find all about vitro fertilization procedure

Find how in vitro fertilization procedure works ivf, risks of in vitro procedure, implantation failure, embryo transfer, the risks and complications of ivf, ivf success rate.

     The first test taken when it comes to analyzing human sperm is the spermatogram, a simple procedure, the basic test in the evaluation of the infertile man.
     The clinical investigation of the infertile couple had a rapid development when it comes to masculine infertility, this fact shads a new light upon human sperm analysis; which due to modern medicine innovations receives more accuracy, precision and credibility.
     What has usually been called human sperm analysis or the traditional spermatogram which assumed a subjective estimation of sperm concentration, motility and morphology gradually has gone out of use in the modern infertility clinics. It has been replaced with alternative procedures, the daily home auto evaluation of the patient’s semen, followed by other elaborated new methods, applied with the aid of precise technical equipment, which can provide an accurate evaluation of the semen conventional characteristics, and, measure other parameters which estimate the functional status and sperm’ capacity, the functional parameters of the accessory sexual glands and seminiferous tubules epithelium.
     In nowadays medicine there are specific methods used in human sperm analysis and those tests are crucial in the diagnosis and investigation of the infertile couple. Spermatogram scientific bases were established by McLeod, (1942) McLeod and Gold (1953), Eliason (1971) and Helliga (1949, 1976) and the pioneer techniques they used led to the modern methods. The precision of a spermatogram lies in the amount of time assigned, physician’s correct and intense work, quality control, and in the biochemical and functional tests, that establish the sperm’ fertilizing potential. An accurate spermatogram contributes to the differentiation of fertile men, subfertile men and infertile men, and it can underline particular cases of infertility and contribute substantially to choosing an appropriate treatment which could elevate the chances of success.
     Furthermore we shall analyze the methods used in human semen collection. It is known that human semen contains a mixture of sperm, testicular and epididymal secretions, mixed with prostate, seminal vesicles and bulboa-urethral glands secretions known as the ejaculate.
     The collection and analysis of the human semen must follow standard procedures in order to provide the necessary information about patient’s infertility.
     The basic instructions related to semen collection were given by the World Health Organization (1987); also the patient must receive clear written instructions about semen collection.
     First of all the semen must be collected after minimum 48 hours and maximum of 7 days of sexual abstinence; then for the initial evaluation two semen samples must be collected; the interval between the two analysis will be of minimum 7 days and maximum 3 months, if the two tests show different results, a third test has to be made, though if the first test is normal further investigation isn’t imperiously necessary.
     The semen has to be preferably collected in an additional room of the laboratory, if not it has to be brought there in maximum one hour after its collection. Also it is preferably to be collected in a small plastic or glass container after masturbation. The container has to be checked first, for toxic substances which could prejudice sperm quality and kept at body temperature. If the spermatogram is accompanied by a bacteriologic test, the patient has to pee before semen collection and wash his hands and penis.
     Normal condoms aren’t recommended because they can interfere with the sperm viability. In particular cases custom made condoms can be used, also coitus interruptus shouldn’t be used because, this method can lead to the loss of the first part of the ejaculate, usually the part with the highest sperm concentration, besides the pH of the vaginal fluids can trigger semen bacteriologic contamination. In the case of an incomplete ejaculation the sample shall not be used because it can provide a false result.
     Those who analyze and maneuver the sample have to be aware of the fact that usually semen conveys viruses like HIV, hepatic virus, herpetic virus etc, thus the sample has to be handled with great care for their personal safety.

Add A Comment

You must be logged in to post a comment.